4th Course on Optical Microscopy Imaging for Biosciences

	Monday, June 18
9:00	Registration and Reception
9:30	Light microscopy overview Paula Sampaio
10:00	Light and Image Formation António Pereira
11:00	Coffee break
11:30	The light microscope anatomy and contrast techniques Paula Sampaio
13:00	Lunch
14:30	Sample Preparation and Labeling Techniques Elsa Logarinho
15:30	Fluorescent probes and proteins Claudia Pereira
16:30	Break
17:00	Practical session - Exploration of light microscope anatomy, setup Koller ilumination, Brigthfield, Phase contrast, DIC and Fluorescence

	Tuesday, June 19
9:30	Information extraction form digital images - An introduction to the basic tools Andre Marçal
11:00	Coffee break
11:30	Digital image aquisition David Castrillo
12:30	TIRF microscopy Jaume Boix
13:00	Lunch
14:30	Optical section and Confocal microscopy Nathalie Garin
15:45	Super-resolution microscopy Nathalie Garin
16:30	Break
17:00	Practical session - Laser Scanning Confocal Microscope setup and image aquisition settings

	Wednesday, June 20
10:00	Image visualization and analysis with Imaris software Delisa Garcia
11:30	Coffee break
12:00	Practical session - Preparation of samples
13:00	Lunch
14:30	Live cell imaging Paula Sampaio
15:30	Cell based assays – new ways in microscopy Ulf Räedler
16:30	Break
17:00	Practical session - Hands on widefield and confocal microscopes

	Thursday, June 21
9:30	Molecular imaging: FRAP, PA, PC & FRET Stefan Terjung
11:00	Coffee break
11:30	Fluorescence Lifetime Imaging Microscopy: Principles and Applications in Biosciences Rodrigo Almeida
12:15	High-content analysis Mark Harvey
13:15	Lunch
15:00	Image processing and analysis with ImageJ/Fiji Stefan Terjung & Paula Sampaio
16:30	Break
17:00	Practical session - Hands on widefield and confocal microscopes

	Friday, June 22
9:30	Live cell imaging assays to study cytokinesis in the early C. elegans embryo Ana Carvalho
10:10	Intravital imaging of malaria pre-erythrocytic phase Joana Tavares
10:50	Axonspotting: from transport to cytoskeleton dynamics Monica Sousa
11:30	Coffee break
12:00	High Content/Throughput Microscopy to Study Golgi Biogenesis and Membrane Traffic in Mammalian Cells Renier Pepperkok
13:00	Lunch
14:30	Imaging dendritic spines in hippocampus: A confocal microscopy approach Diana Rodrigues
15:10	3D development imaging Gabriel Martins
15:50	Applications of advanced fluorescence microscopy to the study of virus particle formation Daniela Ribeiro
16:30	Break
17:00	Results presentations and open discussion
18:30	Final remarks and certificates

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www.ibmc.up.pt