Determining the topology of peroxisomal proteins using protease protection assays - Francisco, T.; Dias, A.F.; Pedrosa, A.G.; Grou, C.P.; Rodrigues, T.A.; Azevedo, J.E. (Methods in Molecular Biology, 2017)

The peroxisomal matrix protein translocon is a large cavity-forming protein assembly into which PEX5 protein enters to release its cargo - Dias, A.F.; Rodrigues, T.A.; Pedrosa, A.G.; Barros-Barbosa, A.; Francisco, T.; Azevedo, J.E. (Journal of Biological Chemistry, 2017)

Protein transport into peroxisomes: Knowns and unknowns - Francisco, T.; Rodrigues, T.A.; Dias, A.F.; Barros-Barbosa, A.; Bicho, D.; Azevedo, J.E. (BioEssays, 2017)

A cell-free organelle-based in vitro system for studying the peroxisomal protein import machinery - Rodrigues, T.A.; Francisco, T.; Dias, A.F.; Pedrosa, A.G.; Grou, C.P.; Azevedo, J.E. (Nature Protocols, 2016)

Evaluation of the activity and substrate specificity of the human SENP family of SUMO proteases - Mendes, A.V.; Grou, C.P.; Azevedo, J.E.; Pinto, M.P. (Biochimica et Biophysica Acta - Molecular Cell Research, 2016)

The first minutes in the life of a peroxisomal matrix protein - Dias, A.F.; Francisco, T.; Rodrigues, T.A.; Grou, C.P.; Azevedo, J.E. (Biochimica et Biophysica Acta - Molecular Cell Research, 2016)

The de novo synthesis of ubiquitin: Identification of deubiquitinases acting on ubiquitin precursors - Grou, C.P.; Pinto, M.P.; Mendes, A.V.; Domingues, P.; Azevedo, J.E. (Scientific Reports, 2015)

The peroxisomal protein import machinery displays a preference for monomeric substrates - Freitas, M.O.; Francisco, T.; Rodrigues, T.A.; Lismont, C.; Domingues, P.; Pinto, M.P.; Grou, C.P.; Fransen, M.; Azevedo, J.E. (Open Biology, 2015)

The Apoptogenic Toxin AIP56 Is a Metalloprotease A-B Toxin that Cleaves NF-κb P65 - Silva, D.S.; Pereira, L.M.G.; Moreira, A.R.; Ferreira-Da-Silva, F.; Brito, R.M.; Faria, T.Q.; Zornetta, I.; Montecucco, C.; Oliveira, P.; Azevedo, J.E.; Pereira, P.J.B.; Macedo-Ribeiro, S.; et al. (PLoS Pathogens, 2013)

A cargo-centered perspective on the PEX5 receptor-mediated peroxisomal protein import pathway - Francisco, T.; Rodrigues, T.A.; Freitas, M.O.; Grou, C.P.; Carvalho, A.F.; Sá-Miranda, C.; Pinto, M.P.; Azevedo, J.E. (Journal of Biological Chemistry, 2013)

Caspase-1 and IL-1β Processing in a Teleost Fish - Reis, M.I.R.; do Vale, A.; Pereira, P.J.B.; Azevedo, J.E.; dos Santos, N.M.S. (PLoS ONE, 2012)

Heat shock induces a massive but differential inactivation of SUMO-specific proteases - Pinto, M.P.; Carvalho, A.F.; Grou, C.P.; Rodríguez-Borges, J. E.; Sá-Miranda, C.; Azevedo, J.E. (Biochimica et Biophysica Acta - Molecular Cell Research, 2012)

Identification of ubiquitin-specific protease 9X (USP9X) as a deubiquitinase acting on ubiquitin-peroxin 5 (PEX5) thioester conjugate - Grou, C.P.; Francisco, T.; Rodrigues, T.A.; Freitas, M.O.; Pinto, M.P.; Carvalho, A.F.; Domingues, P.; Wood, S. A.; Rodríguez-Borges, J. E.; Sá-Miranda, C.; Fransen, M.; Azevedo, J.E. (Journal of Biological Chemistry, 2012)

High-Yield Expression in Escherichia coli and Purification of Mouse Ubiquitin-Activating Enzyme E1 - Carvalho, A.F.; Pinto, M.P.; Grou, C.P.; Vitorino, R.; Domingues, P.; Yamao, F.; Sá-Miranda, C.; Azevedo, J.E. (Molecular Biotechnology, 2011)

PEX5 protein binds monomeric catalase blocking its tetramerization and releases it upon binding the N-terminal domain of PEX14 - Freitas, M.O.; Francisco, T.; Rodrigues, T.A.; Alencastre, I.S.; Pinto, M.P.; Grou, C.P.; Carvalho, A.F.; Fransen, M.; Sá-Miranda, C.; Azevedo, J.E. (Journal of Biological Chemistry, 2011)

The cytosolic domain of PEX3, a protein involved in the biogenesis of peroxisomes, binds membrane lipids - Pinto, M.P.; Grou, C.P.; Fransen, M.; Sá-Miranda, C.; Azevedo, J.E. (Biochimica et Biophysica Acta - Molecular Cell Research, 2009)

Mapping the cargo protein membrane translocation step into the PEX5 cycling pathway - Alencastre, I.S.; Rodrigues, T.A.; Grou, C.P.; Fransen, M.; Sá-Miranda, C.; Azevedo, J.E. (Journal of Biological Chemistry, 2009)

The peroxisomal protein import machinery - A case report of transient ubiquitination with a new flavor - Grou, C.P.; Carvalho, A.F.; Pinto, M.P.; Alencastre, I.S.; Rodrigues, T.A.; Freitas, M.O.; Francisco, T.; Sá-Miranda, C.; Azevedo, J.E. (Cellular and Molecular Life Sciences, 2009)

A nonsense mutation in the LIMP-2 gene associated with progressive myoclonic epilepsy and nephrotic syndrome - Balreira, A.; Gaspar, P.; Caiola, D.; Chaves, J.; Beirão, I.; Lima, J.L.; Azevedo, J.E.; Miranda, M.C.S. (Human Molecular Genetics, 2008)

Chemical chaperones reduce endoplasmic reticulum stress and prevent mutant HFE aggregate formation - de Almeida, S.F.; Picarote, G.; Fleming, J.V.; Carmo-Fonseca, M.; Azevedo, J.E.; De Sousa, M. (Journal of Biological Chemistry, 2007)

Functional characterization of two missense mutations in Pex5p-C11S and N526K - Carvalho, A.F.; Grou, C.P.; Pinto, M.P.; Alencastre, I.S.; Costa-Rodrigues, J.; Fransen, M.; Sá-Miranda, C.; Azevedo, J.E. (Biochimica et Biophysica Acta - Molecular Cell Research, 2007)

Proteomics characterization of mouse kidney peroxisomes by tandem mass spectrometry and protein correlation profiling - Wiese, S.; Gronemeyer, T.; Ofman, R.; Kunze, M.; Grou, C.P.; Almeida, J.A.; Eisenacher, M.; Stephan, C.; Hayen, H.; Schollenberger, L.; Korosec, T.; Waterham, H.R.; et al. (Molecular and Cellular Proteomics, 2007)

Stimulation of an unfolded protein response impairs MHC class I expression - de Almeida, S.F.; Fleming, J.V.; Azevedo, J.E.; Carmo-Fonseca, M.; De Sousa, M. (Journal of Immunology, 2007)

Ubiquitination of mammalian Pex5p, the peroxisomal import receptor - Carvalho, A.F.; Pinto, M.P.; Grou, C.P.; Alencastre, I.S.; Fransen, M.; Sá-Miranda, C.; Azevedo, J.E. (Journal of Biological Chemistry, 2007)

The import competence of a peroxisomal membrane protein is determined by Pex19p before the docking step - Pinto, M.P.; Grou, C.P.; Alencastre, I.S.; Oliveira, M.E.; Sá-Miranda, C.; Fransen, M.; Azevedo, J.E. (Journal of Biological Chemistry, 2006)

The N-terminal half of the peroxisomal cycling receptor Pex5p is a natively unfolded domain - Carvalho, A.F.; Costa-Rodrigues, J.; Correia, I.; Costa Pessoa, J.; Faria, T.Q.; Martins, C.L.; Fransen, M.; Sá-Miranda, C.; Azevedo, J.E. (Journal of Molecular Biology, 2006)

Pex14p, more than just a docking protein - Azevedo, J.E.; Schliebs, W. (Biochimica et Biophysica Acta - Molecular Cell Research, 2006)

AIP56, a novel plasmid-encoded virulence factor of Photobacterium damselae subsp. piscicida with apoptogenic activity against sea bass macrophages and neutrophils - do Vale, A.; Silva, M.T.; dos Santos, N.M.S.; Nascimento, D.S.; Reis-Rodrigues, P.; Costa-Ramos, C.; Ellis, A.E.; Azevedo, J.E. (Molecular Microbiology, 2005)

HFE cross-talks with the MHC class I antigen presentation pathway - de Almeida, S.F.; Carvalho, I.F.; Cardoso, C.S.; Cordeiro, J.V.; Azevedo, J.E.; Neefjes, J.; De Sousa, M. (Blood, 2005)

Pex5p, the peroxisomal cycling receptor, is a monomeric non-globular protein - Costa-Rodrigues, J.; Carvalho, A.F.; Fransen, M.; Hambruch, E.; Schliebs, W.; Sá-Miranda, C.; Azevedo, J.E. (Journal of Biological Chemistry, 2005)

Probing substrate-induced conformational alterations in adrenoleukodystrophy protein by proteolysis - Guimarães, C.P.; Sá-Miranda, C.; Azevedo, J.E. (Journal of Human Genetics, 2005)

Mouse liver PMP70 and ALDP: Homomeric interactions prevail in vivo - Guimarães, C.P.; Domingues, P.; Aubourg, P.; Fouquet, F.; Pujol, A.; Jimenez-Sanchez, G.; Sá-Miranda, C.; Azevedo, J.E. (Biochimica et Biophysica Acta - Molecular Basis of Disease, 2004)

The N terminus of the peroxisomal cycling receptor, Pex5p, is required for redirecting the peroxisome-associated peroxin back to the cytosol - Costa-Rodrigues, J.; Carvalho, A.F.; Gouveia, A.M.; Fransen, M.; Sá-Miranda, C.; Azevedo, J.E. (Journal of Biological Chemistry, 2004)

Protein translocation across the peroxisomal membrane - Azevedo, J.E.; Costa-Rodrigues, J.; Guimarães, C.P.; Oliveira, M.E.; Sá-Miranda, C. (Cell Biochemistry and Biophysics, 2004)