14-16 APRIL 2015 | IBMC

THE COURSE COVERS:
- fundamentals of flow cytometry
- flow cytometry experimental design
- set up and acquisition on a flow cytometer
- analysis and interpretation of the acquired data
- DNA analysis and cell death applications in flow cytometry

 

14th APRIL 2015

9:00-9:30 Introduction to the flow cytometry course
Catarina Leitão, IBMC, Portugal

9:30-10:15 Fluorescence
Catarina Leitão, IBMC, Portugal

10:15-10:30 Coffee break

10:30-12:00 Components of Flow Cytometry
Rui Gardner, IGC, Portugal

12:00-13:00 Lunch

13:00-15:00 Multicolour Flow Cytometry
Rui Gardner, IGC, Portugal

15:00-15:30 Coffee break

15:30-17:00 Experimental setup
Catarina Leitão, IBMC, Portugal and Rui Gardner, IGC, Portugal

 

15th APRIL 2015

9:00-10:15 Hands-on flow cytometer
Catarina Leitão, IBMC, Portugal and Rui Gardner, IGC, Portugal

10:15-10:30 Coffee break

10:30-12:00 Hands-on flow cytometer
Catarina Leitão, IBMC, Portugal and Rui Gardner, IGC, Portugal

12:00-13:15 Lunch

13:15-15:00 Data analysis and statistics
Catarina Leitão, IBMC, Portugal and Rui Gardner, IGC, Portugal

15:00-15:30 Coffee break

15:30-17:00 Data analysis and statistics
Catarina Leitão, IBMC, Portugal and Rui Gardner, IGC, Portugal

 

16th APRIL 2015

09:00-09:30 Short introductory talk (DNA analysis and proliferation)
Derek Davies, The Francis Crick Institute, London, United Kingdom

09:30-10:30 Viability and simple DNA histogram
Derek Davies, The Francis Crick Institute, London, United Kingdom

10:30-11:00 Coffee Break

11:00-12:00 Vital DNA staining with Hoechst 33342; BrdU staining; mpm2 staining
Derek Davies, The Francis Crick Institute, London, United Kingdom

12:00-12:30 Data analysis of morning’s samples (FlowJo)
Derek Davies, The Francis Crick Institute, London, United Kingdom

12:30-13:15 Lunch

13:15-13:45 Short Introductory talk (Apoptosis)
Derek Davies, The Francis Crick Institute, London, United Kingdom

13:45-15:00 Apoptosis by Annexin, TMRE and YO-PRO-1
Derek Davies, The Francis Crick Institute, London, United Kingdom

15:00-15:30 Coffee Break

15:30-14:15 Apoptosis by Sub G1 and TUNEL
Derek Davies, The Francis Crick Institute, London, United Kingdom

14:15-16:45 Data analysis of afternoon’s samples (FlowJo)
Derek Davies, The Francis Crick Institute, London, United Kingdom

16:45-17:00 Q&A and wrap up
Derek Davies, The Francis Crick Institute, London, United Kingdom

 

The number of participants is restricted to 10 places.

 

 

	Until March 2nd	Until April 2nd
I3S members	110 euros	130 euros
Others	220 euros	250 euros

Registration fee includes participation on the workshop (Flow Cytometry workshop – DNA analysis and Cell death), coffee breaks, lunches and course material. 

The number of participants is restricted to 10 places.

 

Registrations are closed.

If you want to stay on waiting list or receive information about future editions of the course, please fill the following form: http://www.ibmc.up.pt/eventos/registrationnew.php?eid=65

 

YOUTH HOSTEL in OPORTO
Webpage: http://www.pousadasjuventude.pt

SEMINÁRIO de VILAR 

Webpage: http://www.seminariodevilar.pt

HF TUELA PORTO ***

Webpage: http://www.hfhotels.com/hf-tuela-porto

HOTEL DOURO ***
Webpage: http://www.hoteldouro.pt

HOTEL EUROSTARS DAS ARTES **** 
Webpage: http://www.eurostarsdasartes.com

 

Catarina Leitão | Advanced Flow Cytometry Unit, IBMC – course coordinator 
E-mail: catarina.leitao@ibmc.up.pt

Mariana Resende | Microbiology and Immunology of Infection
E-mail: mrsilva@ibmc.up.pt 

Andreia Costa | IBMC training center, IBMC
E-mail: training@ibmc.up.pt

Rita Matos | Events management office, IBMC
E-mail: rita.matos@ibmc.up.pt