instituto de biologia molecular e celular | institute for molecular and cell biology
The 3rd Course on High Content Screening and Image Analysis for Biosciences is a comprehensive and intensive course that aims to provide the basis of image-based screening. It is designed to graduate students, early-career investigators and everyone willing to enter in the multiplex era. The course includes lectures given by specialists in the field and hands-on sessions with HCS instruments and open-source software for image and data analysis.
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- High content screening
- High content imaging systems
- High throughput screening
- Lab automation
- Target and drug discovery
- High content image analysis
- Data visualization and analysis
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Tuesday, 6 June |
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09:00 | Registration and Reception | |
09:30 |
High Content Imaging and Screening | André Maia, i3S |
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10:15 |
Target and Drug Discovery | António Pombinho, i3S |
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11:00 | Coffee-break | |
11:30 |
Light microscopy overview | Paula Sampaio, i3S |
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12:00 | Light and Image Formation | António Pereira, i3S | |
13:00 | Lunch (included) | |
14:00 | High Content Imaging Systems | Soren Prag, Zeiss | |
15:00 | High Content Imaging Tools | TBA | |
16:00 | Coffee-break | |
16:30 | Lab Automation and Liquid Handling | tba | |
Wednesday, 7 June |
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09:30 | Lab sessions overview | André Maia, i3S | |
10:00 |
LAB SESSION 1 |
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11:00 | Coffee-break | |
11:30 | Data Analysis in High Content Microscopy | Hugo Botelho, BioISI, FCUL | |
12:30 |
Software installation for Lab Session 2 |
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13:00 | Lunch (included) | |
14:00 | LAB SESSION 2 | |
15:30 | Coffee-break | |
16:00 | LAB SESSION 2 | |
17:30 | LAB SESSION 3 | |
Thursday, 8 June |
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09:30 | LAB SESSION 4 | |
11:00 | Coffee-break | |
11:30 |
TBA | Janos Kriston-Vizi, MRC, UCL |
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13:00 | Lunch (included) | |
14:00 | LAB SESSION 5 | |
15:30 | Coffee-break | |
16:00 | LAB SESSION 6 | |
Friday, 9 June |
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09:30 | An introduction to Cell Profiler and Cell Profiler Analyst | Romain Guiet, EPFL | |
10:00 | Software installation for Lab Session 7 | |
10:15 | LAB SESSION 7 | |
11:30 | Coffee-break | |
12:00 | A synthetic lethal screen for dynein pathway genes in C.elegans | Helder Rocha, i3S | |
13:00 | Lunch (included) | |
14:00 | LAB SESSION 7 | |
16:00 | Coffee-break | |
16:30 | Experimental work discussion and Q/A | |
17:00 |
Closing remarks and certificates |
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LAB SESSION 1 and 3 – Assay preparation and liquid handling. Three screening example experiments will be performed: a) Drug screening and hit finding using high content imaging tools and instruments
b) Viability screen with a homogeneous assay
c) Time-lapse microscopy screen
LAB SESSION 4 – Assay preparation (HC imaging tools), Multi-mode microplate reader, Automated microscopy
LAB SESSION 5 – Imaging processing workflow by ImageJ
LAB SESSION 6 – Image/Data acquisition
LAB SESSION 7 – Image Analysis with Cell Profiler and Cell Profiler Analyst
LAB SESSION 2 – Data Analysis with HTM Explorer
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Liquidator 96 >>
JANUS >>
Multidrop Combi >>
IN Cell Analyzer 2000 >>
Cell Discoverer 7, sponsored by Zeiss/Taper >>
Synergy 2 >>
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André Maia, i3S, Porto
António Pombinho, i3S, Porto
Paula Sampaio, i3S, Porto
António Pereira, i3S, Porto
Soren Prag, Zeiss
Hugo Botelho, BioISI, Lisboa
Janos Kriston-Vizi, MRC, London
Romain Guiet, EPFL, Lausanne
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Before 15 May 2017 | i3S members – 180€
Before 15 May 2017 | General registration - 225€
After 15 May 2017 | i3S members or General registration – 250€
Registration fee includes coffee breaks, lunches and course materials.
Limited number of participants (15)
Registration Deadline: 23 May 2017
Please make the payment only after the registration confirmation.
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Walking distance to i3S
Hotel Ibis São João **
Rua Dr. Plácido Costa, 4200-450 Porto
Website: http://www.ibis.com/pt/hotel-3227-ibis-porto-sao-joao
Telephone: (+351) 225 513 100 | E-mail: H3227@ACCOR.COM
Walking distance: 10 minutes
Accessible by subway from Porto city center
Pão de Açúcar Hotel ***
Rua do Almada, 262, 4050-032 Porto
Website: http://www.paodeacucarhotel.pt/
Telephone: (+351) 222 002 425 / 222 011 589 | E-mail: info@paodeacucarhotel.pt
Average time: 15 minutes
Quality Inn Porto
Praça da Batalha, 127-130, 4000-102 Porto
Website: http://www.continentalhotels.eu/site/hotels/view/24.html
Telephone: (+351) 223 392 300 | E-mail: quality.batalha@grupo-continental.com
Average time: 25 minutes
Trindade Porto Hotel ****
Rua de Camões, n.º 129 / 131, 4000-144 Porto
Website: http://www.portotrindadehotel.com/formularios.aspx?id=1
Telephone: (+351) 222 061 520 | E-mail: geral@portotrindadehotel.com
Average time: 15 minutes
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André Maia – BioSciences Screening Unit
António Pombinho - BioSciences Screening Unit
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
Telf: +351 220 408 821 | E-mail: andre.maia@i3s.up.pt
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CONTACTS
Advanced Training Unit | E-mail: training@i3s.up.pt
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SPONSORS
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