Course on High Content Screening and Image Analysis for BioSciences

The 3rd Course on High Content Screening and Image Analysis for Biosciences is a comprehensive and intensive course that aims to provide the basis of image-based screening. It is designed to graduate students, early-career investigators and everyone willing to enter in the multiplex era. The course includes lectures given by specialists in the field and hands-on sessions with HCS instruments and open-source software for image and data analysis.

Poster download >>

 

3rd Course on High Content Screening and Image Analysis for BioSciences
6-9 June 2017 | i3S, Porto

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COURSE CONTENTS

- High content screening
- High content imaging systems
- High throughput screening
- Lab automation
- Target and drug discovery
- High content image analysis
- Data visualization and analysis

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PROGRAMME

Tuesday, 6 June

     
09:00   Registration and Reception
09:30  

High Content Imaging and Screening | André Maia, i3S

10:15  

Target and Drug Discovery | António Pombinho, i3S

     
11:00   Coffee-break
     
11:30  

Light microscopy overview | Paula Sampaio, i3S

12:00   Light and Image Formation | António Pereira, i3S
     
13:00   Lunch (included)
     
14:00   High Content Imaging Systems | Soren Prag, Zeiss
15:00   High Content Imaging Tools | TBA
     
16:00   Coffee-break
     
16:30    Lab Automation and Liquid Handling | tba
     

Wednesday, 7 June

     
09:30   Lab sessions overview | André Maia, i3S
     
10:00  

LAB SESSION 1

     
11:00   Coffee-break
     
11:30   Data Analysis in High Content Microscopy | Hugo Botelho, BioISI, FCUL
12:30  

Software installation for Lab Session 2

     
13:00   Lunch (included)
     
14:00   LAB SESSION 2
     
15:30   Coffee-break
     
16:00   LAB SESSION 2
     
17:30   LAB SESSION 3
     

Thursday, 8 June

     
09:30   LAB SESSION 4
     
11:00   Coffee-break
     
11:30  

TBA | Janos Kriston-Vizi, MRC, UCL

     
13:00   Lunch (included)
     
14:00   LAB SESSION 5
     
15:30   Coffee-break
     
16:00   LAB SESSION 6
     

Friday, 9 June

     
09:30   An introduction to Cell Profiler and Cell Profiler Analyst | Romain Guiet, EPFL
10:00   Software installation for Lab Session 7
     
10:15   LAB SESSION 7
     
11:30   Coffee-break
     
12:00   A synthetic lethal screen for dynein pathway genes in C.elegans | Helder Rocha, i3S
     
13:00   Lunch (included)
     
14:00   LAB SESSION 7
     
16:00   Coffee-break
     
16:30   Experimental work discussion and Q/A
     
17:00  

Closing remarks and certificates

 

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LAB SESSIONS INFO

LAB SESSION 1 and 3 – Assay preparation and liquid handling. Three screening example experiments will be performed:     a) Drug screening and hit finding using high content imaging tools and instruments
b) Viability screen with a homogeneous assay
c) Time-lapse microscopy screen

LAB SESSION 4 – Assay preparation (HC imaging tools), Multi-mode microplate reader, Automated microscopy

LAB SESSION 5 – Imaging processing workflow by ImageJ

LAB SESSION 6 – Image/Data acquisition

LAB SESSION 7 – Image Analysis with Cell Profiler and Cell Profiler Analyst

LAB SESSION 2 – Data Analysis with HTM Explorer

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EQUIPMENT

Liquidator 96 >> 
JANUS >> 
Multidrop Combi >> 
IN Cell Analyzer 2000 >> 
Cell Discoverer 7, sponsored by Zeiss/Taper >> 
Synergy 2 >> 

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SPEAKERS

André Maia, i3S, Porto
António Pombinho, i3S, Porto
Paula Sampaio, i3S, Porto
António Pereira, i3S, Porto
Soren Prag, Zeiss
Hugo Botelho, BioISI, Lisboa
Janos Kriston-Vizi, MRC, London
Romain Guiet, EPFL, Lausanne

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FEES

Before 15 May 2017 | i3S members – 180€
Before 15 May 2017 | General registration - 225€
After 15 May 2017 | i3S members or General registration – 250€

Registration fee includes coffee breaks, lunches and course materials.
Limited number of participants (15)

Registration Deadline: 23 May 2017

Registration >>

Please make the payment only after the registration confirmation.

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ACCOMMODATION SUGGESTIONS

Walking distance to i3S

Hotel Ibis São João **
Rua Dr. Plácido Costa, 4200-450 Porto
Website: http://www.ibis.com/pt/hotel-3227-ibis-porto-sao-joao
Telephone: (+351) 225 513 100 | E-mail: H3227@ACCOR.COM
Walking distance: 10 minutes

Accessible by subway from Porto city center

Pão de Açúcar Hotel ***
Rua do Almada, 262, 4050-032 Porto
Website: http://www.paodeacucarhotel.pt/
Telephone: (+351) 222 002 425 / 222 011 589 | E-mail: info@paodeacucarhotel.pt
Average time: 15 minutes

Quality Inn Porto
Praça da Batalha, 127-130, 4000-102 Porto
Website: http://www.continentalhotels.eu/site/hotels/view/24.html
Telephone: (+351) 223 392 300 | E-mail: quality.batalha@grupo-continental.com
Average time: 25 minutes

Trindade Porto Hotel ****
Rua de Camões, n.º 129 / 131, 4000-144 Porto
Website: http://www.portotrindadehotel.com/formularios.aspx?id=1
Telephone: (+351) 222 061 520 | E-mail: geral@portotrindadehotel.com
Average time: 15 minutes

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COURSE COORDINATION

André Maia – BioSciences Screening Unit
António Pombinho - BioSciences Screening Unit
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
Telf: +351 220 408 821 | E-mail: andre.maia@i3s.up.pt

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CONTACTS

Advanced Training Unit | E-mail: training@i3s.up.pt

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SPONSORS

            

 

                            

 

             

 

  

 


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