EMBO Practical Course | Biomolecular interaction analysis 2016: From molecules to cells

EMBO Practical Course
Biomolecular interaction analysis 2016: From molecules to cells

7 – 11 November 2016 | i3S, Porto, Portugal

A comprehensive and integrated characterization of molecular interactions is key to the understanding of life processes at a molecular level. Due to their complexity the study of binding events requires the gathering of data from multiple methods in order to build a complete picture of their function. The 3rd Edition of this practical course aims at a multidisplinary approach to the study of molecular interactions from and in biological systems, integrating biochemical/biophysical methodologies with live microscopy techniques.

We will cover a range of methods used in the kinetics and thermodynamics characterization of binding events, such as:

Isothermal Titration Calorimetry (ITC)

  • Surface Plasmon Resonance (SPR)
  • Microscale Thermophoresis (MST)
  • Surface Acoustic Wave (SAW)
  • Electro-Switchable Biosurfaces (switchSENSE)

The bridge to in vivo will be crossed with the introduction of advanced light microscopy techniques, e.g.:

  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Lifetime Imaging Microscopy (FLIM)

to study the dynamic interactions of proteins within the living cell.

More details about the course and applications at http://events.embo.org/16-biomolecule/index.html

Updates on the course at http://bit.ly/28P3xwL


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