Introduction to digital image analysis with ImageJ /Fiji

With the advent of digital era, nowadays images are multidimensional numerical data that can be processed and analyzed quantitatively to extract more information. ImageJ/Fiji open source software ( is a powerful tool available to process and analyze digital images. Moreover, it also allow the automation of image analysis with custom macros and plugins. With this course we intend to give a general introduction to ImageJ/Fiji, show how to use it to extract quantitative data and how to write simple macros. This course will be focused in the analysis of light microscopy images, but is targeted to all people engaged in research, that wants to start using ImageJ/Fiji for image analysis in general.

i3S | Porto | 19-21 September, 2017

COORDINATOR: Paula Sampaio, i3S – IBMC

Principles, techniques and ethics of digital bioimage processing. Basic commands of ImageJ/Fiji software. Working with multidimensional (2D, 3D, time-lapse, position) bioimages. Enhancement and processing of images. Extract quantitative numerical data. Design of simple workflows for automation of image analysis with ImageJ macros. Creation of figures for publication. Participants are invited to submit in advance an analysis problem / data of their interest that might be addressed in the course as an individual mini-project (with the help of the tutors).

The participant should bring a personal laptop for the sessions


i3S members: 75 EUR
Externals: 100 EUR

Registration Deadline: 31 August 2017

Limited number of participants: 25

Registrations are now closed. If you wish to be placed in the waiting list, please send an e-mail to 




Home | Site Map | Contacts | Credits | Privacy & Cookies | Intranet | Social Networks |

rua alfredo allen, 208, 4200-135 porto - portugal | tel +351 220 408 800 | email: | © copyright 2010 ibmc