CID - Chromosome Instability & Dynamics Lab | Publications
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Lab publications

  • Ferreira, LT, and Maiato, H. (2021). Prometaphase. Semin Cell Dev Bio, Sep;117:52-61. [Pubmed] [Reprint request]

  • Cheeseman, L.P., and Maiato, H. (2021). Chromosomal instability: Stretching the role of checkpoint silencing. Current Biology 31, R386-R389. [Pubmed] [Reprint request]

  • Ferreira, L.T., Logarinho, E., Macedo, J.C., Maia, A.R.R. & Maiato, H. SOGA1 and SOGA2/MTCL1 are CLASP-interacting proteins required for faithful chromosome segregation in human cells. Chromosome Research (2021). [Pubmed] [Reprint request]

  • Lopes, D., and Maiato, H. (2020). The Tubulin Code in Mitosis and Cancer. Cells 9. [Pubmed] [Reprint request]

  • Ferreira, L. T., B. Orr, G. Rajendraprasad, A. J. Pereira, C. Lemos, J. T. Lima, C. Guasch Boldú, J. G. Ferreira, M. Barisic and H. Maiato (2020). α-Tubulin detyrosination impairs mitotic error correction by suppressing MCAK centromeric activity. Journal of Cell Biology 219(4). [Pubmed] [Reprint request]

  • Girão, H., N. Okada, T. A. Rodrigues, A. O. Silva, A. C. Figueiredo, Z. Garcia, T. Moutinho-Santos, I. Hayashi, J. E. Azevedo, S. Macedo-Ribeiro and H. Maiato (2020). “CLASP2 binding to curved microtubule tips promotes flux and stabilizes kinetochore attachments.” J Cell Biol 219(2). [Pubmed] [Reprint request]

  • Almeida, A. C., D. Drpic, N. Okada, J. Bravo, M. Madureira and H. Maiato (2020). Functional Dissection of Mitosis Using Immortalized Fibroblasts from the Indian Muntjac, a Placental Mammal with Only Three Chromosomes. Methods Mol Biol 2101: 247-266. [Pubmed] [Reprint request]

  • Girão, H. and H. Maiato (2020). Measurement of Microtubule Half-Life and Poleward Flux in the Mitotic Spindle by Photoactivation of Fluorescent Tubulin. Methods Mol Biol 2101: 235-246. [Pubmed] [Reprint request]

  • Afonso, O., C. M. Castellani, L. P. Cheeseman, J. G. Ferreira, B. Orr, L. T. Ferreira, J. J. Chambers, E. Morais-de-Sa, T. J. Maresca and H. Maiato (2019). Spatiotemporal control of mitotic exit during anaphase by an aurora B-Cdk1 crosstalk. Elife 8. [Pubmed] [Reprint request]

  • Orr, B. and H. Maiato (2019). No chromosome left behind: The importance of metaphase alignment for mitotic fidelity. J Cell Biol 218(4): 1086-1088. [Pubmed] [Reprint request]

  • Pereira, A., M. Sousa, A. C. Almeida, L. T. Ferreira, A. R. Costa, M. Novais-Cruz, C. Ferras, M. M. Sousa, P. Sampaio, M. Belsley and H. Maiato (2019). Coherent-hybrid STED: high contrast sub-diffraction imaging using a bi-vortex depletion beam. Opt Express 27(6): 8092-8111. [Pubmed] [Reprint request]

  • Almeida AC, Maiato H. (2018) Chromokinesins. Curr Biol. 28(19):R1131-R1135. [Pubmed] [Reprint request]

  • Novais-Cruz M, Alba Abad M, van IJcken WF, Galjart N, Jeyaprakash AA, Maiato H, Ferrás C. (2018) Mitotic progression, arrest, exit or death relies on centromere structural integrity, rather than de novo transcription. Elife. 7. pii: e36898.. [Pubmed] [Reprint request]

  • Ferreira LT, Figueiredo AC, Orr B, Lopes D, Maiato H. (2018) Dissecting the role of the tubulin code in mitosis. Methods Cell Biol. 144:33-74. . [Pubmed] [Reprint request]

  • Drpic D, Almeida AC, Aguiar P, Renda F, Damas J, Lewin HA, Larkin DM, Khodjakov A, Maiato H. (2018) Chromosome Segregation Is Biased by Kinetochore Size. Curr Biol. 28(9):1344-1356.e5. [Pubmed] [Reprint request]

  • Akhmanova A, Maiato H. (2017) Closing the tubulin detyrosination cycle. Science, 358(6369):1381-1382. [Pubmed] [Reprint request]

  • Figueiredo AC, Maiato H. (2017) Kinetochore regulation: Let there be light. Nat Chem Biol. 13(9):1058-1059. [Pubmed] [Reprint request]

  • Maiato H, Pereira AJ. (2017) Cell Division: NuMA Bears the Load in the Spindle. Curr Biol. 27(15):R765-R767. [Pubmed] [Reprint request]

  • Maiato H, Ferrás C. (2017) Actin divides to conquer. Science; 357(6353):756-757. [Pubmed] [Reprint request]

  • Maiato H, Gomes AM, Sousa F, Barisic M. (2017) Mechanisms of Chromosome Congression during Mitosis. Biology (Basel); 6(1). pii: E13. [Pubmed] [Reprint request]

  • Barisic M, Maiato H. (2016) The Tubulin Code: A Navigation System for Chromosomes during Mitosis. Trends Cell Biol. pii: S0962-8924(16)30071-X. [Pubmed] [Reprint request]

  • Maiato H. (2016) Miguel Mota (1922-2016)-the kinetochore engine(er). Chromosome Res. 24(2):281-3. [Pubmed] [Reprint request]

  • Afonso O, Figueiredo AC, Maiato H. (2016) Late mitotic functions of Aurora kinases. Chromosoma. 2016 Apr 22. [Epub ahead of print] Review. [Pubmed] [Reprint request]

  • Pereira AJ, Aguiar P, Belsley M, Maiato H. (2016) Inducible fluorescent speckle microscopy. J Cell Biol. 2016 Jan 18;212(2):245-55. [Pubmed] [Reprint request]

  • Schweizer N, Maiato H. (2015) Membrane-based mechanisms of mitotic spindle assembly. Commun Integr Biol. 8(6):e1112473. [Pubmed] [Reprint request]

  • Drpic D, Pereira AJ, Barisic M, Maresca TJ, Maiato H. (2015) Polar Ejection Forces Promote the Conversion from Lateral to End-on Kinetochore-Microtubule Attachments on Mono-oriented Chromosomes. [Pubmed] [Reprint request]

  • Barisic M, Maiato H. (2015) Dynein prevents erroneous kinetochore-microtubule attachments in mitosis. Cell Cycle. 2015 Sep 23:0. [Epub ahead of print]. [Pubmed] [Reprint request]

  • Schweizer N, Pawar N, Weiss M, Maiato H. (2015) An organelle-exclusion envelope assists mitosis and underlies distinct molecular crowding in the spindle region. J Cell Biol. 210(5):695-704. [Pubmed] [Reprint request]

  • Barisic M, Maiato H. (2015) Cracking the (tubulin) code of mitosis. Oncotarget. 2015 Aug 14;6(23):19356-7. [Pubmed] [Reprint request]

  • Barisic M, Silva e Sousa R, Tripathy SK, Magiera MM, Zaytsev AV, Pereira AL, Janke C, Grishchuk EL, Maiato H. (2015) Mitosis. Microtubule detyrosination guides chromosomes during mitosis. Science. 348(6236):799-803. [Pubmed] [Reprint request]

  • Schweizer N, Weiss M, Maiato H. (2014) The dynamic spindle matrix. Curr Opin Cell Biol. 28:1-7. [Pubmed] [Reprint request]

  • Ferreira JG, Pereira AL, Maiato H. (2014) Microtubule plus-end tracking proteins and their roles in cell division. Int Rev Cell Mol Biol. 309:59-140. [Pubmed] [Reprint request]

  • Moutinho-Santos T, Maiato H. (2014) Plk1 puts a (Has)pin on the mitotic histone code. EMBO Rep. 15(3):203-4. [Pubmed] [Reprint request]

  • Maiato H, Logarinho E. (2014) Mitotic spindle multipolarity without centrosome amplification. Nat Cell Biol. 16(5):386-94. [Pubmed] [Reprint request]

  • Afonso O, Matos I, Pereira AJ, Aguiar P, Lampson MA, Maiato H. (2014) Feedback control of chromosome separation by a midzone Aurora B gradient. Science. 345(6194):332-6. [Pubmed] [Reprint request]

  • Barisic M, Aguiar P, Geley S, Maiato H. (2014) Kinetochore motors drive congression of peripheral polar chromosomes by overcoming random arm-ejection forces. Nat Cell Biol. 16(12):1249-56. [Pubmed] [Reprint request]

  • Maiato H, Afonso O, Matos I. (2014) A chromosome separation checkpoint: A midzone Aurora B gradient mediates a chromosome separation checkpoint that regulates the anaphase-telophase transition. Bioessays. 37(3):257-66. [Pubmed] [Reprint request]

  • Afonso O, Matos I, Maiato H. (2014) Spatial control of the anaphase-telophase transition. Cell Cycle. 13(19):2985-6. [Pubmed] [Reprint request]

  • Maiato H, Barral Y. (2013) Unbiased about chromosome segregation: give me a mechanism and I will make you “immortal”. Chromosome Res. 21(3):189-91. [Pubmed] [Reprint request]

  • Drpic D, Barisic M, Pinheiro D, Maiato H. (2013) Selective tracking of template DNA strands after induction of mitosis with unreplicated genomes (MUGs) in Drosophila S2 cells. Chromosome Res. 21(3):329-37. [Pubmed] [Reprint request]

  • Maia AR, Zhu X, Miller P, Gu G, Maiato H, Kaverina I. (2013) Modulation of Golgi-associated microtubule nucleation throughout the cell cycle. Cytoskeleton (Hoboken). 70(1):32-43. [Pubmed] [Reprint request]

  • Maiato H. (2013) Helder Maiato: hot (+)TIPs on mitosis. Interview by Caitlin Sedwick. J Cell Biol. 202(5):722-3. [Pubmed] [Reprint request]

  • Ferreira JG, Pereira AJ, Akhmanova A, Maiato H. (2013) Aurora B spatially regulates EB3 phosphorylation to coordinate daughter cell adhesion with cytokinesis. J Cell Biol. 201(5):709-24. [Pubmed] [Reprint request]

  • Moutinho-Pereira S, Stuurman N, Afonso O, Hornsveld M, Aguiar P, Goshima G, Vale RD, Maiato H. (2013) Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells. Proc Natl Acad Sci U S A. 110(49):19808-13. [Pubmed] [Reprint request]

  • Schweizer N, Ferrás C, Kern DM, Logarinho E, Cheeseman IM, Maiato H. (2013) Spindle assembly checkpoint robustness requires Tpr-mediated regulation of Mad1/Mad2 proteostasis. J Cell Biol. 203(6):883-93. [Pubmed] [Reprint request]

  • Barisic M, Pereira AJ, Maiato H. (2012) Fluorescent speckle microscopy in cultured cells. Methods Enzymol. 504:147-61. [Pubmed] [Reprint request]

  • Maia AR, Garcia Z, Kabeche L, Barisic M, Maffini S, Macedo-Ribeiro S, Cheeseman IM, Compton DA, Kaverina I, Maiato H. (2012) Cdk1 and Plk1 mediate a CLASP2 phospho-switch that stabilizes kinetochore-microtubule attachments. J Cell Biol. 199(2):285-301. [Pubmed] [Reprint request]

  • Logarinho E, Maffini S, Barisic M, Marques A, Toso A, Meraldi P, Maiato H. (2012) CLASPs prevent irreversible multipolarity by ensuring spindle-pole resistance to traction forces during chromosome alignment. Nat Cell Biol. 14(3):295-303. [Pubmed] [Reprint request]

  • Pereira AJ, Maiato H. (2012) Maturation of the kinetochore-microtubule interface and the meaning of metaphase. Chromosome Res. 20(5):563-77. [Pubmed] [Reprint request]

  • Maiato H, Logarinho E. (2011) Motor-dependent and -independent roles of CENP-E at kinetochores: the cautionary tale of UA62784. Chem Biol. 18(6):679-80. [Pubmed] [Reprint request]

  • Maia AR, Maiato H. (2011) Aurora Mitochondrialis drives fission during mitosis. Dev Cell. 21(3):387-8. [Pubmed] [Reprint request]

  • Pereira AJ, Maiato H. (2010) Improved kymography tools and its applications to mitosis. Methods. 51(2):214-9. [Pubmed] [Reprint request]

  • Maiato H. (2010) Mitosis: wisdom, knowledge, and information. Cell Mol Life Sci. 67(13):2141-3. [Pubmed] [Reprint request]

  • Maiato H, Lince-Faria M. (2010) The perpetual movements of anaphase. Cell Mol Life Sci. 67(13):2251-69. [Pubmed] [Reprint request]

  • Moutinho-Pereira S, Matos I, Maiato H. (2010) Drosophila S2 cells as a model system to investigate mitotic spindle dynamics, architecture, and function. Methods Cell Biol. 97:243-57. [Pubmed] [Reprint request]

  • Moutinho-Pereira S, Debec A, Maiato H. (2009) Microtubule cytoskeleton remodeling by acentriolar microtubule-organizing centers at the entry and exit from mitosis in Drosophila somatic cells. Mol Biol Cell. 20(11):2796-808. [Pubmed] [Reprint request]

  • Lince-Faria M, Maffini S, Orr B, Ding Y, Cláudia Florindo, Sunkel CE, Tavares A, Johansen J, Johansen KM, Maiato H. (2009) Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator. J Cell Biol. 184(5):647-57. [Pubmed] [Reprint request]

  • Maffini S, Maia AR, Manning AL, Maliga Z, Pereira AL, Junqueira M, Shevchenko A, Hyman A, Yates JR 3rd, Galjart N, Compton DA, Maiato H. (2009) Motor-independent targeting of CLASPs to kinetochores by CENP-E promotes microtubule turnover and poleward flux. Curr Biol. 19(18):1566-72. [Pubmed] [Reprint request]

  • Pereira AJ, Matos I, Lince-Faria M, Maiato H. (2009) Dissecting mitosis with laser microsurgery and RNAi in Drosophila cells. Methods Mol Biol. 545:145-64. [Pubmed] [Reprint request]

  • Matos I, Pereira AJ, Lince-Faria M, Cameron LA, Salmon ED, Maiato H. (2009) Synchronizing chromosome segregation by flux-dependent force equalization at kinetochores. J Cell Biol. 186(1):11-26. [Pubmed] [Reprint request]

  • Pereira AL, Pereira AJ, Maia AR, Drabek K, Sayas CL, Hergert PJ, Lince-Faria M, Matos I, Duque C, Stepanova T, Rieder CL, Earnshaw WC, Galjart N, Maiato H. (2006) Mammalian CLASP1 and CLASP2 cooperate to ensure mitotic fidelity by regulating spindle and kinetochore function. Mol Biol Cell. 17(10):4526-42. [Pubmed] [Reprint request]


Steblyanko, Y., Rajendraprasad, G., Osswald, M., Eibes, S., Jacome, A., Geley, S., Pereira, A.J., Maiato, H., and Barisic, M. (2020). Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins. EMBO J 39, e105432. [Pubmed]

Nunes, V., M. Dantas, D. Castro, E. Vitiello, I. Wang, N. Carpi, M. Balland, M. Piel, P. Aguiar, H. Maiato and J. G. Ferreira (2020). Centrosome-nuclear axis repositioning drives the assembly of a bipolar spindle scaffold to ensure mitotic fidelity. Mol Biol Cell: mbcE20010047. [Pubmed]

Vitiello E, Moreau P, Nunes V, Mettouchi A, Maiato H, Ferreira JG, Wang I, Balland M. (2019) Acto-myosin force organization modulates centriole separation and PLK4 recruitment to ensure centriole fidelity. Nat Commun. 3;10(1):52. [Pubmed]

Jana SC, Mendonça S, Machado P, Werner S, Rocha J, Pereira A, Maiato H, Bettencourt-Dias M. (2018) Differential regulation of transition zone and centriole proteins contributes to ciliary base diversity. Nat Cell Biol. 20(8):928-941. [Pubmed]

Moura M, Osswald M, Leça N, Barbosa J, Pereira AJ, Maiato H, Sunkel CE, Conde C. (2017) Protein Phosphatase 1 inactivates Mps1 to ensure efficient Spindle Assembly Checkpoint silencing. Elife. May 2;6. pii: e25366. [Pubmed]

Silva AM, Osório DS, Pereira AJ, Maiato H, Pinto IM, Rubinstein B, Gassmann R, Telley IA, Carvalho AX. Robust gap repair in the contractile ring ensures timely completion of cytokinesis. J Cell Biol. 215(6):789-799. [Pubmed]

Su KC, Barry Z, Schweizer N, Maiato H, Bathe M, Cheeseman IM. (2016) A Regulatory Switch Alters Chromosome Motions at the Metaphase-to-Anaphase Transition. Cell Rep. 17(7):1728-1738.  [Pubmed]

Orticello M, Fiore M, Totta P, Desideri M, Barisic M, Passeri D, Lenzi J, Rosa A, Orlandi A, Maiato H, Del Bufalo D, Degrassi F. (2015) N-terminus-modified Hec1 suppresses tumour growth by interfering with kinetochore-microtubule dynamics. Oncogene 34(25):3325-35. [Pubmed]

Tan CH, Gasic I, Huber-Reggi SP, Dudka D, Barisic M, Maiato H, Meraldi P. (2015) The equatorial position of the metaphase plate ensures symmetric cell divisions. Elife. 2015 Jul 18;4. [Pubmed]

Xavier de Carvalho A, Maiato H, Maia AF, Ribeiro SA, Pontes P, Bickmore W, Earnshaw WC, Sambade C. (2015) Reed-Sternberg cells form by abscission failure in the presence of functional Aurora B kinase. PLoS One. 10(5):e0124629. [Pubmed]

Vitiello E, Ferreira JG, Maiato H, Balda MS, Matter K. (2014) The tumour suppressor DLC2 ensures mitotic fidelity by coordinating spindle positioning and cell-cell adhesion. Nat Commun. 5:5826. [Pubmed]

Bertalan Z, La Porta CA, Maiato H, Zapperi S. (2014) Conformational mechanism for the stability of microtubule-kinetochore attachments. Biophys J. 107(2):289-300. [Pubmed]

Conde C, Osswald M, Barbosa J, Moutinho-Santos T, Pinheiro D, Guimarães S, Matos I, Maiato H, Sunkel CE. (2013) Drosophila Polo regulates the spindle assembly checkpoint through Mps1-dependent BubR1 phosphorylation. EMBO J. 32(12):1761-77. [Pubmed]

Lecland N, Debec A, Delmas A, Moutinho-Pereira S, Malmanche N, Bouissou A, Dupré C, Jourdan A, Raynaud-Messina B, Maiato H, Guichet A. (2013) Establishment and mitotic characterization of new Drosophila acentriolar cell lines from DSas-4 mutant. Biol Open. 2(3):314-23. [Pubmed]

Drabek K, Gutiérrez L, Vermeij M, Clapes T, Patel SR, Boisset JC, van Haren J, Pereira AL, Liu Z, Akinci U, Nikolic T, van Ijcken W, van den Hout M, Meinders M, Melo C, Sambade C, Drabek D, Hendriks RW, Philipsen S, Mommaas M, Grosveld F, Maiato H, Italiano JE Jr, Robin C, Galjart N. (2012) The microtubule plus-end tracking protein CLASP2 is required for hematopoiesis and hematopoietic stem cell maintenance. Cell Rep. 2(4):781-8. [Pubmed]

Wandke C, Barisic M, Sigl R, Rauch V, Wolf F, Amaro AC, Tan CH, Pereira AJ, Kutay U, Maiato H, Meraldi P, Geley S. (2012) Human chromokinesins promote chromosome congression and spindle microtubule dynamics during mitosis. J Cell Biol. 198(5):847-63. [Pubmed]

Yao C, Rath U, Maiato H, Sharp D, Girton J, Johansen KM, Johansen J. (2012) A nuclear-derived proteinaceous matrix embeds the microtubule spindle apparatus during mitosis. Mol Biol Cell. 23(18):3532-41. [Pubmed]

Olszak AM, van Essen D, Pereira AJ, Diehl S, Manke T, Maiato H, Saccani S, Heun P. (2011) Heterochromatin boundaries are hotspots for de novo kinetochore formation. Nat Cell Biol. 13(7):799-808. [Pubmed]

Manning AL, Bakhoum SF, Maffini S, Correia-Melo C, Maiato H, Compton DA. (2010) CLASP1, astrin and Kif2b form a molecular switch that regulates kinetochore-microtubule dynamics to promote mitotic progression and fidelity. EMBO J. 29(20):3531-43. [Pubmed]

Ding Y, Yao C, Lince-Faria M, Rath U, Cai W, Maiato H, Girton J, Johansen KM, Johansen J. (2009) Chromator is required for proper microtubule spindle formation and mitosis in Drosophila. Dev Biol. 334(1):253-63. [Pubmed]

Reis R, Feijão T, Gouveia S, Pereira AJ, Matos I, Sampaio P, Maiato H, Sunkel CE (2009). Dynein and mast/orbit/CLASP have antagonistic roles in regulating kinetochore-microtubule plus-end dynamics. J Cell Sci. 122(Pt 14):2543-53. [Pubmed]

Coelho PA, Queiroz-Machado J, Carmo AM, Moutinho-Pereira S, Maiato H, Sunkel CE (2008). Dual role of topoisomerase II in centromere resolution and aurora B activity. PLoS Biol. 2008 Aug 26;6(8):e207. [Pubmed]

Williams B, Leung G, Maiato H, Wong A, Li Z, Williams EV, Kirkpatrick C, Aquadro CF, Rieder CL, Goldberg ML. (2007) Mitch a rapidly evolving component of the Ndc80 kinetochore complex required for correct chromosome segregation in Drosophila. J Cell Sci. 120(Pt 20):3522-33. [Pubmed]

Efimov A, Kharitonov A, Efimova N, Loncarek J, Miller PM, Andreyeva N, Gleeson P, Galjart N, Maia AR, McLeod IX, Yates JR 3rd, Maiato H, Khodjakov A, Akhmanova A, Kaverina I. (2007) Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network. Dev Cell. 12(6):917-30. [Pubmed]