Advanced Light Microscopy - Publications

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Output including data acquired at ALM in last 5 years:

Vieira M, Gomes JR, Saraiva MJ. Transthyretin Induces Insulin-like Growth Factor I Nuclear Translocation Regulating Its Levels in the Hippocampus. Mol Neurobiol. 2014 Aug 2. [Epub ahead of print] PMID: 25084758

Afonso O, Matos I, Pereira AJ, Aguiar P, Lampson MA, Maiato H. Feedback control of chromosome separation by a midzone Aurora B gradient. Science. 2014;345:332-6.

Mar FM, Simões AR, Leite S, Morgado MM, Santos TE, Rodrigo IS, Teixeira CA, Misgeld T, Sousa MM. CNS axons globally increase axonal transport after peripheral conditioning. J Neurosci. 2014;34:5965-70.

Pereira CL, Gonçalves RM, Peroglio M, Pattappa G, D'Este M, Eglin D, Barbosa MA, Alini M, Grad S. The effect of hyaluronan-based delivery of stromal cell-derived factor-1 on the recruitment of MSCs in degenerating intervertebral discs. Biomaterials. 2014;35:8144-53.

Fonseca KB, Gomes DB, Lee K, Santos SG, Sousa A, Silva EA, Mooney DJ, Granja PL, Barrias CC. Injectable MMP-sensitive alginate hydrogels as hMSC delivery systems. Biomacromolecules. 2014;15:380-90.

Teixeira CA, Miranda CO, Sousa VF, Santos TE, Malheiro AR, Solomon M, Maegawa GH, Brites P, Sousa MM. Early axonal loss accompanied by impaired endocytosis, abnormal axonal transport, and decreased microtubule stability occur in the model of Krabbe's disease. Neurobiol Dis. 2014;66:92-103.

Maia FR, Lourenço AH, Granja PL, Gonçalves RM, Barrias CC. Effect of cell density on mesenchymal stem cells aggregation in RGD-alginate 3D matrices under osteoinductive conditions. Macromol Biosci. 2014;14:759-71.

Maia FR, Fonseca KB, Rodrigues G, Granja PL, Barrias CC. Matrix-driven formation of mesenchymal stem cell-extracellular matrix microtissues on soft alginate hydrogels. Acta Biomater. 2014;10:3197-208.

Santos-Gomes GM, Rodrigues A, Teixeira F, Carreira J, Alexandre-Pires G, Carvalho S, Santos-Mateus D, Martins C, Vale-Gato I, Marques C, Tomás AM. Immunization with the Leishmania infantum recombinant cyclophilin protein 1 confers partial protection to subsequent parasite infection and generates specific memory T cells. Vaccine. 2014 Mar 5;32:1247-53.

Silva-Gomes S, Santos AG, Caldas C, Silva CM, Neves JV, Lopes J, Carneiro F, Rodrigues PN, Duarte TL. Transcription factor NRF2 protects mice against dietary iron-induced liver injury by preventing hepatocytic cell death. J Hepatol. 2014;60:354-61.

Guerreiro SG, Oliveira MJ, Barbosa MA, Soares R, Granja PL. Neonatal Human Dermal Fibroblasts Immobilized in RGD-Alginate Induce Angiogenesis. Cell Transplant. 2014;23:945-57.

Gonçalves IC, Magalhães A, Fernandes M, Rodrigues IV, Reis CA, Martins MC. Bacterial-binding chitosan microspheres for gastric infection treatment and prevention. Acta Biomater. 2013;9:9370-8

Laranjeira MS, Fernandes MH, Monteiro FJ. Response of monocultured and co-cultured human microvascular endothelial cells and mesenchymal stem cells to macroporous granules of nanostructured-hydroxyapatite agglomerates. J Biomed Nanotechnol. 2013;9:1594-606.

Vasconcelos DP, Fonseca AC, Costa M, Amaral IF, Barbosa MA, Águas AP, Barbosa JN. Macrophage polarization following chitosan implantation. Biomaterials. 2013;34:9952-9.

Amaral IF, Neiva I, Ferreira da Silva F, Sousa SR, Piloto AM, Lopes CD, Barbosa MA, Kirkpatrick CJ, Pêgo AP. Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII7-10). Acta Biomater. 2013;9:5643-52

Rodrigues SC, Salgado CL, Sahu A, Garcia MP, Fernandes MH, Monteiro FJ. Preparation and characterization of collagen-nanohydroxyapatite biocomposite scaffolds by cryogelation method for bone tissue engineering applications. J Biomed Mater Res A. 2013;101:1080-94.

Moreira S, Bishopp A, Carvalho H, Campilho A. AHP6 inhibits cytokinin signaling to regulate the orientation of pericycle cell division during lateral root initiation. PLoS One. 2013;8:e56370.

Miranda CO, Teixeira CA, Sousa VF, Santos TE, Liz MA, Marques AM, Pinto-do-Ó P, Sousa MM. Primary bone marrow mesenchymal stromal cells rescue the axonal phenotype of Twitcher mice. Cell Transplant. 2014 Feb;23:239-52.

Feijão T, Afonso O, Maia AF, Sunkel CE. Stability of kinetochore-microtubule attachment and the role of different KMN network components in Drosophila. Cytoskeleton (Hoboken). 2013;70:661-75.

Misumi Y, Ando Y, Gonçalves NP, Saraiva MJ. Fibroblasts endocytose and degrade transthyretin aggregates in transthyretin-related amyloidosis. Lab Invest. 2013;93:911-20.

Ferreira JG, Pereira AJ, Akhmanova A, Maiato H. Aurora B spatially regulates EB3 phosphorylation to coordinate daughter cell adhesion with cytokinesis. J Cell Biol. 2013;201:709-24.

Conde C, Osswald M, Barbosa J, Moutinho-Santos T, Pinheiro D, Guimarães S, Matos I, Maiato H, Sunkel CE. Drosophila Polo regulates the spindle assembly checkpoint through Mps1-dependent BubR1 phosphorylation. EMBO J. 2013;32:1761-77.

Fernandes AS, Castro A, Videira A. Reduced glutathione export during programmed cell death of Neurospora crassa. Apoptosis. 2013;18:940-8.

Silva-Gomes S, Appelberg R, Larsen R, Soares MP, Gomes MS. Heme catabolism by heme oxygenase-1 confers host resistance to Mycobacterium infection. Infect Immun. 2013;81:2536-45.

Morais da Silva S, Moutinho-Santos T, Sunkel CE. A tumor suppressor role of the Bub3 spindle checkpoint protein after apoptosis inhibition. J Cell Biol. 2013;201:385-93.

Batista AR, Sena-Esteves M, Saraiva MJ. Hepatic production of transthyretin L12P leads to intracellular lysosomal aggregates in a new somatic transgenic mouse model. Biochim Biophys Acta. 2013;1832:1183-93.

Marinho J, Martins T, Neto M, Casares F, Pereira PS. The nucleolar protein Viriato/Nol12 is required for the growth and differentiation progression activities of the Dpp pathway during Drosophila eye development. Dev Biol. 2013;377:154-65.

Monjardino P, Rocha S, Tavares AC, Fernandes R, Sampaio P, Salema R, da Câmara Machado A. Development of flange and reticulate wall ingrowths in maize (Zea mays L.) endosperm transfer cells. Protoplasma. 2013;250:495-503.

Rodrigues SC, Salgado CL, Sahu A, Garcia MP, Fernandes MH, Monteiro FJ. Preparation and characterization of collagen-nanohydroxyapatite biocomposite scaffolds by cryogelation method for bone tissue engineering applications. Biomed Mater Res A. 2013;101A:1080–1094.

Amaral IF, Neiva I, Ferreira da Silva F, Sousa SR, Piloto AM, Lopes CD, Barbosa MA, Kirkpatrick CJ, Pego AP. Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII(7-10)). Acta Biomater. 2013;9:5643–5652.

Paiva D, Martõn-Molina A, Cardoso I, Quesada-Perez M, Pereira MC and Rocha S. The effect of a fluorinated cholesterol derivative on the stability and physical properties of cationic DNA vectors. Soft Matter, 2013;9:401-09.

Costa-Rodrigues J, Carmo SC, Silva JC, Fernandes MH. Inhibition of human in vitro osteoclastogenesis by Equisetum arvense. Cell Prolif. 2012;45:566-76.

Santos C, Gomes PS, Duarte JA, Franke RP, Almeida MM, Costa ME, Fernandes MH. Relevance of the sterilization-induced effects on the properties of different hydroxyapatite nanoparticles and assessment of the osteoblastic cell response. J R Soc Interface. 2012 ;9:3397-410.

Bessa Pereira C, Gomes PS, Costa-Rodrigues J, Almeida Palmas R, Vieira L, Ferraz MP, Lopes MA, Fernandes MH. Equisetum arvense hydromethanolic extracts in bone tissue regeneration: in vitro osteoblastic modulation and antibacterial activity. Cell Prolif. 2012;45:386-96.

Costa-Rodrigues J, Martins EG, Fernandes MH. Induced osteoclastogenesis by fluoroquinolones in unstimulated and stimulated human osteoclast precursor cells. Bone. 2012;51:17-27.

da Costa, DS, Pires RA, Frias AM, Reis RL and Pashkuleva, IJ. Sulfonic groups induce formation of filopodia in mesenchymal stem cells. Mater. Chem., 2012;22:7172-7178

Costa-Rodrigues J, Fernandes A, Lopes MA, Fernandes MH. Hydroxyapatite surface roughness: complex modulation of the osteoclastogenesis of human precursor cells. Acta Biomater. 2012;8:1137-45.

Costa-Rodrigues J, Moniz KA, Teixeira MR, Fernandes MH. Variability of the paracrine-induced osteoclastogenesis by human breast cancer cell lines. J Cell Biochem. 2012;113:1069-79.

Maciel J, Oliveira MI, Gonçalves RM, Barbosa MA. The effect of adsorbed fibronectin and osteopontin on macrophage adhesion and morphology on hydrophilic and hydrophobic model surfaces. Acta Biomater. 2012;8:3669-77.

Laranjeira MS, Fernandes MH, Monteiro FJ. Reciprocal induction of human dermal microvascular endothelial cells and human mesenchymal stem cells: time-dependent profile in a co-culture system. Cell Prolif. 2012;45:320-34.

Grenho L, Manso MC, Monteiro FJ, Ferraz MP. Adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa onto nanohydroxyapatite as a bone regeneration material. J Biomed Mater Res A. 2012;100:1823-30.

Bonekamp NA, Sampaio P, de Abreu FV, LŸers GH, Schrader M. Transient complex interactions of Mammalian peroxisomes without exchange of matrix or membrane marker proteins. Traffic. 2012;13:960-78.

Silva AM, Tavares J, Silvestre R, Ouaissi A, Coombs GH, Cordeiro-da-Silva A. Characterization of Leishmania infantum thiol-dependent reductase 1 and evaluation of its potential to induce immune protection. Parasite Immunol. 2012;34:345-50.

Gonçalves NP, Oliveira H, Pêgo AP, Saraiva MJ. A novel nanoparticle delivery system for in vivo targeting of the sciatic nerve: impact on regeneration. Nanomedicine (Lond). 2012;71167-1180.

Almeida CR, Vasconcelos DP, Gonçalves RM, Barbosa MA. Enhanced mesenchymal stromal cell recruitment via natural killer cells by incorporation of inflammatory signals in biomaterials. J R Soc Interface. 2012;9:261-71.

Logarinho E, Maffini S, Barisic M, Marques A, Toso A, Meraldi P, Maiato H. CLASPs prevent irreversible multipolarity by ensuring spindle-pole resistance to traction forces during chromosome alignment. Nat Cell Biol. 2012;14:295-303.

Moutinho-Santos T, Conde C, Sunkel CE.  POLO ensures chromosome bi-orientation by preventing and correcting erroneous chromosome-spindle attachments. J Cell Sci. 2012;125:576-83.

Costa-Rodrigues J, Fernandes A, Fernandes MH. Spontaneous and induced osteoclastogenic behaviour of human peripheral blood mononuclear cells and their CD14(+) and CD14(-) cell fractions. Cell Prolif. 2011;44:410-9.

Costa-Rodrigues J, Fernandes A, Fernandes MH. Reciprocal osteoblastic and osteoclastic modulation in co-cultured MG63 osteosarcoma cells and human osteoclast precursors. J Cell Biochem. 2011;112:3704-13.

Lopes PP, Ferreira BJ, Gomes PS, Correia RN, Fernandes MH, Fernandes MH. Silicate and borate glasses as composite fillers: a bioactivity and biocompatibility study. J Mater Sci Mater Med. 2011;22:1501-10.

Costa-Rodrigues J, Fernandes MH. Paracrine-mediated differentiation and activation of human haematopoietic osteoclast precursor cells by skin and gingival fibroblasts. Cell Prolif. 2011;44:264-73.

Carvalho L, Alberto NJ, Gomes PS, Nogueira RN, Pinto JL, Fernandes MH. In the trail of a new bio-sensor for measuring strain in bone: osteoblastic biocompatibility. Biosens Bioelectron. 2011;26:4046-52.

Costa-Rodrigues J, Teixeira CA, Fernandes MH. Paracrine-mediated osteoclastogenesis by the osteosarcoma MG63 cell line: is RANKL/RANK signalling really important? Clin Exp Metastasis. 2011;28:505-14.

de Gomes PS, Figueiral MH, Fernandes MH, Scully C. Cytotoxicity of denture adhesives. Clin Oral Investig. 2011;15:885-93.

Fonseca KB, Bidarra SJ, Oliveira MJ, Granja PL, Barrias CC. Molecularly designed alginate hydrogels susceptible to local proteolysis as three-dimensional cellular microenvironments. Acta Biomater. 2011;7:1674-82.

Bidarra SJ, Barrias CC, Fonseca KB, Barbosa MA, Soares RA, Granja PL. Injectable in situ crosslinkable RGD-modified alginate matrix for endothelial cells delivery. Biomaterials. 2011;32:7897-904.

Antunes RF, Brandão C, Maia M, Arosa FA. Red blood cells release factors with growth and survival bioactivities for normal and leukemic T cells. Immunol Cell Biol. 2011;89:111-21.

Costa-Ramos C, Vale A, Ludovico P, Dos Santos NM, Silva MT. The bacterial exotoxin AIP56 induces fish macrophage and neutrophil apoptosis using mechanisms of the extrinsic and intrinsic pathways. Fish Shellfish Immunol. 2011;30:173-81.

Santos SD, Lambertsen KL, Clausen BH, Akinc A, Alvarez R, Finsen B, Saraiva MJ. CSF transthyretin neuroprotection in a mouse model of brain ischemia. J Neurochem. 2010;115:1434-44.

Marinho J, Casares F, Pereira PS. The Drosophila Nol12 homologue viriato is a dMyc target that regulates nucleolar architecture and is required for dMyc-stimulated cell growth. Development. 2011;138:349-57.

Pinto PA, Henriques T, Freitas MO, Martins T, Domingues RG, Wyrzykowska PS, Coelho PA, Carmo AM, Sunkel CE, Proudfoot NJ, Moreira A. RNA polymerase II kinetics in polo polyadenylation signal selection. EMBO J. 2011;30:2431-44.

Miranda CO, Teixeira CA, Liz MA, Sousa VF, Franquinho F, Forte G, Di Nardo P, Pinto-Do-î P, Sousa MM. Systemic delivery of bone marrow-derived mesenchymal stromal cells diminishes neuropathology in a mouse model of Krabbe's disease. Stem Cells. 2011;29:1738-51.

Castro H, Teixeira F, Romao S, Santos M, Cruz T, Florido M, Appelberg R, Oliveira P, Ferreira-da-Silva F, Tom‡s AM. Leishmania mitochondrial peroxiredoxin plays a crucial peroxidase-unrelated role during infection: insight into its novel chaperone activity. PLoS Pathog. 2011;7:e1002325.

Brites P, Ferreira AS, Ferreira da Silva T, Sousa VF, Malheiro AR, Duran M, Waterham HR, Baes M, Wanders RJ. Alkyl-glycerol rescues plasmalogen levels and pathology of ether-phospholipid deficient mice. PLoS One. 2011;6:e28539.

Pires LR, Oliveira H, Barrias CC, Sampaio P, Pereira AN, Maiato HL, Sim Es SR, P Go AP. Imidazole-grafted chitosan-mediated gene delivery: in vitro study on transfection, intracellular trafficking and degradation. Nanomedicine (Lond). 2011;6:1499-512.

Orr B, Sunkel CE. Drosophila CENP-C is essential for centromere identity. Chromosoma. 2011;120:83-96.

Teixeira L, Botelho AS, Mesquita SD, Correia A, Cerca F, Costa R, Sampaio P, Castro AG, Vilanova M. Plasmacytoid and conventional dendritic cells are early producers of IL-12 in Neospora caninum-infected mice. Immunol Cell Biol. 2010;88:79-86.

Pinto JP, Dias V, Zoller H, Porto G, Carmo H, Carvalho F, de Sousa M. Hepcidin messenger RNA expression in human lymphocytes. Immunology. 2010;130:217-30.

Maule C, Gonçalves H, Mendonça C, Sampaio P, Esteves da Silva JC, Jorge P. Wavelength encoded analytical imaging and fiber optic sensing with pH sensitive CdTe quantum dots. Talanta. 2010;80:1932-8.

Marinho J, Casares F, Pereira PS. The Drosophila Nol12 homologue viriato is a dMyc target that regulates nucleolar architecture and is required for dMyc-stimulated cell growth. Development. 2011;138:349-57.

Maia AF, Feij‹o T, Vromans MJ, Sunkel CE, Lens SM. Aurora B kinase cooperates with CENP-E to promote timely anaphase onset. Chromosoma. 2010;119:405-13

Magalh‹es J, Santos SD, Saraiva MJ. _B-crystallin (HspB5) in familial amyloidotic polyneuropathy. Int J Exp Pathol. 2010;91:515-21.

Laranjeira MS, Fernandes MH, Monteiro FJ. Innovative macroporous granules of nanostructured-hydroxyapatite agglomerates: bioactivity and osteoblast-like cell behaviour. J Biomed Mater Res A. 2010;95:891-900.

Gonçalves R, Martins MC, Oliveira MJ, Almeida-Porada G, Barbosa MA. Bioactivity of immobilized EGF on self-assembled monolayers: optimization of the immobilization process. J Biomed Mater Res A. 2010;94:576-85.

Fernandes SS, Nunes A, Gomes AR, de Castro B, Hider RC, Rangel M, Appelberg R, Gomes MS. Identification of a new hexadentate iron chelator capable of restricting the intramacrophagic growth of Mycobacterium avium. Microbes Infect. 2010;12:287-94.

Costa-Rodrigues J, Teixeira CA, Sampaio P, Fernandes MH. Characterisation of the osteoclastogenic potential of human osteoblastic and fibroblastic conditioned media. J Cell Biochem. 2010;109:205-16.

Castro H, Romao S, Carvalho S, Teixeira F, Sousa C, Tom‡s AM. Mitochondrial redox metabolism in trypanosomatids is independent of tryparedoxin activity. PLoS One. 2010;5:e12607.

Bidarra SJ, Barrias CC, Barbosa MA, Soares R, Granja PL. Immobilization of human mesenchymal stem cells within RGD-grafted alginate microspheres and assessment of their angiogenic potential. Biomacromolecules. 2010;11:1956-64.

Reis R, Feij‹o T, Gouveia S, Pereira AJ, Matos I, Sampaio P, Maiato H, Sunkel CE. Dynein and Mast/Orbit/CLASP have antagonistic roles in regulating kinetochore-microtubule plus-end dynamics. J Cell Sci. 2009;122:2543-53.

Oliveira MJ, Costa AM, Costa AC, Ferreira RM, Sampaio P, Machado JC, Seruca R, Mareel M, Figueiredo C. CagA associates with c-Met, E-cadherin, and p120-catenin  in a multiproteic complex that suppresses Helicobacter pylori-induced cell-invasive phenotype. J Infect Dis. 2009;200:745-55.

Moutinho-Pereira S, Debec A, Maiato H. Microtubule cytoskeleton remodeling by  acentriolar microtubule-organizing centers at the entry and exit from mitosis in Drosophila somatic cells. Mol Biol Cell. 2009;20:2796-808.

Matos I, Pereira AJ, Lince-Faria M, Cameron LA, Salmon ED, Maiato H. Synchronizing chromosome segregation by flux-dependent force equalization at kinetochores. J Cell Biol. 2009;186:11-26.

Martins T, Maia AF, Steffensen S, Sunkel CE. Sgt1, a co-chaperone of Hsp90 stabilizes Polo and is required for centrosome organization. EMBO J. 2009;28:234-47.

Maffini S, Maia AR, Manning AL, Maliga Z, Pereira AL, Junqueira M, Shevchenko  A, Hyman A, Yates JR 3rd, Galjart N, Compton DA, Maiato H. Motor-independent targeting of CLASPs to kinetochores by CENP-E promotes microtubule turnover and poleward flux. Curr Biol. 2009;19:1566-72.

Lince-Faria M, Maffini S, Orr B, Ding Y, Cláudia Florindo, Sunkel CE, Tavares  A, Johansen J, Johansen KM, Maiato H. Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator. J Cell Biol. 2009;184:647-57.

Fleming CE, Mar FM, Franquinho F, Saraiva MJ, Sousa MM. Transthyretin internalization by sensory neurons is megalin mediated and necessary for its neuritogenic activity. J Neurosci. 2009 ;29:3220-32.

Ding Y, Yao C, Lince-Faria M, Rath U, Cai W, Maiato H, Girton J, Johansen KM,  Johansen J. Chromator is required for proper microtubule spindle formation and mitosis in Drosophila. Dev Biol. 2009;334:253-63.

Costa-Rodrigues J, Teixeira CA, Sampaio P, Fernandes MH. Characterisation of the osteoclastogenic potential of human osteoblastic and fibroblastic conditioned media. J Cell Biochem. 2010;109:205-16.

Bouissou A, VŽrollet C, Sousa A, Sampaio P, Wright M, Sunkel CE, Merdes A, Raynaud-Messina B. Gamma-Tubulin ring complexes regulate microtubule plus end dynamics. J Cell Biol. 2009;187:327-34.