Selected publications including work done at the Unit:

 

Teixeira F, Castro H, Cruz T, Tse E, Koldewey P, Southworth DR, Tomás AM, Jakob U. Mitochondrial peroxiredoxin functions as crucial chaperone reservoir in  Leishmania infantum. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201419682.

 

Fraga J, Maranha A, Mendes V, Pereira PJ, Empadinhas N, Macedo-Ribeiro S. Structure of mycobacterial maltokinase, the missing link in the essential GlgE-pathway. Sci Rep. 2015 Jan 26;5:8026

 

Santos JA, Alonso-García N, Macedo-Ribeiro S, Pereira PJ. The unique regulation of iron-sulfur cluster biogenesis in a Gram-positive bacterium. Proc Natl Acad Sci U S A. 2014 Jun 3;111(22):E2251-60

 

Pessoa J, Fonseca F, Furini S, Morais-Cabral JH. Determinants of ligand selectivity in a cyclic nucleotide-regulated potassium channel. J Gen Physiol. 2014 Jul;144(1):41-54

 

Leblebici P, Leblebici ME, Ferreira-da-Silva F, Rodrigues AE, Pais LS. Separation of human immunoglobulin G subclasses on a protein A monolith column. J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jul 1;962:89-93.

 

Pereira LM, Pinto RD, Silva DS, Moreira AR, Beitzinger C, Oliveira P, Sampaio P, Benz R, Azevedo JE, dos Santos NM, do Vale A. Intracellular trafficking of AIP56, an NF-κB-cleaving toxin from Photobacterium damselae subsp. piscicida. Infect Immun. 2014 Dec;82(12):5270-85.

 

Santos JA, Alonso-García N, Macedo-Ribeiro S, Pereira PJ. The unique regulation of iron-sulfur cluster biogenesis in a Gram-positive bacterium. Proc Natl Acad Sci U S A. 2014 Jun 3;111(22):E2251-60.

 

Vieira-Pires RS, Szollosi A, Morais-Cabral JH. The structure of the KtrAB potassium transporter. Nature. 2013 Apr 18;496(7445):323-8.

 

Silva DS, Pereira LM, Moreira AR, Ferreira-da-Silva F, Brito RM, Faria TQ, Zornetta I, Montecucco C, Oliveira P, Azevedo JE, Pereira PJ, Macedo-Ribeiro S, do Vale A, dos Santos NM. The apoptogenic toxin AIP56 is a metalloprotease A-B toxin that cleaves NF-κb P65. PLoS Pathog. 2013 Feb;9(2):e1003128.

 

Sá-Moura B, Simões AM, Fraga J, Fernandes H, Abreu IA, Botelho HM, Gomes CM, Marques AJ, Dohmen RJ, Ramos PC, Macedo-Ribeiro S. Biochemical and biophysical characterization of recombinant yeast proteasome maturation factor ump1. Comput Struct Biotechnol J. 2013 Sep 10;7:e201304006.

 

Figueiredo AC, de Sanctis D, Pereira PJ. The tick-derived anticoagulant madanin is processed by thrombin and factor Xa. PLoS One. 2013 Aug 12;8(8):e71866

 

Amaral IF, Neiva I, Ferreira da Silva F, Sousa SR, Piloto AM, Lopes CD, Barbosa MA, Kirkpatrick CJ, Pêgo AP. Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII7-10). Acta Biomater. 2013 Mar;9(3):5643-52.

 

Figueiredo AC, de Sanctis D, Gutiérrez-Gallego R, Cereija TB, Macedo-Ribeiro S, Fuentes-Prior P, Pereira PJ. Unique thrombin inhibition mechanism by anophelin, an anticoagulant from the malaria vector. Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):E3649-58.

 

Castro-Rodrigues AF, Gales L, Saraiva MJ, Damas AM. Structural insights into a zinc-dependent pathway leading to Leu55Pro transthyretin amyloid fibrils. Acta Crystallogr D Biol Crystallogr. 2011 Dec;67(Pt 12):1035-44

 

Marques-Carvalho MJ, Sahoo N, Muskett FW, Vieira-Pires RS, Gabant G, Cadene M, Schönherr R, Morais-Cabral JH. Structural, biochemical, and functional characterization of the cyclic nucleotide binding homology domain from the mouse  EAG1 potassium channel. J Mol Biol. 2012 Oct 12;423(1):34-46

 

Liz MA, Leite SC, Juliano L, Saraiva MJ, Damas AM, Bur D, Sousa MM. Transthyretin is a metallopeptidase with an inducible active site. Biochem J. 2012 May 1;443(3):769-78

 

Carneiro P, Duarte M, Videira A. Disruption of alternative NAD(P)Hdehydrogenases leads to decreased mitochondrial ROS in Neurospora crassa. Free Radic Biol Med. 2012 Jan 15;52(2):402-9.

 

Carneiro P, Duarte M, Videira A. Characterization of apoptosis-related oxidoreductases from Neurospora crassa. PLoS One. 2012;7(3):e34270.

 

Figueiredo AC, Clement CC, Zakia S, Gingold J, Philipp M, Pereira PJ. Rational design and characterization of D-Phe-Pro-D-Arg-derived direct thrombin inhibitors. PLoS One. 2012;7(3):e34354.

 

Pereira CL, Antunes JC, Gonçalves RM, Ferreira-da-Silva F, Barbosa MA. Biosynthesis of highly pure poly-γ-glutamic acid for biomedical applications. J Mater Sci Mater Med. 2012 Apr 25. [Epub ahead of print]

 

Rocha R, Pereira PJ, Santos MA, Macedo-Ribeiro S. Unveiling the structural basis for translational ambiguity tolerance in a human fungal pathogen. Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14091-6

 

Garriga D, Vives-Adrián L, Buxaderas M, Ferreira-da-Silva F, Almeida B, Macedo-Ribeiro S, Pereira PJ, Verdaguer N. Cloning, purification and preliminary crystallographic studies of the 2AB protein from hepatitis A virus. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Oct 1;67(Pt 10):1224-7. .

 

Castro H, Teixeira F, Romao S, Santos M, Cruz T, Flórido M, Appelberg R, Oliveira P, Ferreira-da-Silva F, Tomás AM. Leishmania Mitochondrial Peroxiredoxin Plays a Crucial Peroxidase-Unrelated Role during Infection: Insight into Its Novel Chaperone Activity. PLoS Pathog. 2011 Oct;7(10):e1002325. .

 

Antunes JC, Pereira CL, Molinos M, Ferreira-da-Silva F, Dessı̀ M, Gloria A, Ambrosio L, Gonçalves RM, Barbosa MA. Layer-by-Layer Self-Assembly of Chitosan and Poly(γ-glutamic acid) into Polyelectrolyte Complexes. Biomacromolecules. 2011.

 

Sárkány Z, Ikonen TP, Ferreira-da-Silva F, Saraiva MJ, Svergun D, Damas AM. Solution Structure of the Soluble Receptor for Advanced Glycation End Products (sRAGE). J Biol Chem. 2011 Oct 28;286(43):37525-34.

 

Duarte M, Ferreira-da-Silva F, Lünsdorf H, Junca H, Gales L, Pieper DH, Nunes OC. Gulosibacter molinativorax ON4T molinate hydrolase, a novel cobalt-dependent amidohydrolase. J Bacteriol. 2011

 

Mari SA, Pessoa J, Altieri S, Hensen U, Thomas L, Morais-Cabral JH, Müller DJ. Gating of the MlotiK1 potassium channel involves large rearrangements of the cyclic nucleotide-binding domains. Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20802-7.

 

Empadinhas, N.; Pereira, P.J.B.; Albuquerque, L.; Costa, J.; Sá-Moura, B.; Marques, A.T.; Macedo-Ribeiro, S.; da Costa, M.S. (2011) “Functional and structural characterization of a novel mannosyl-3-phosphoglycerate synthase from Rubrobacter xylanophilus reveals its dual substrate specificity.” Molecular Microbiology 79, 76-93.

 

Costa R, Speretta E, Crowther DC, Cardoso I. Testing the Therapeutic Potential of Doxycycline in a Drosophila melanogaster Model of Alzheimer Disease. J Biol Chem. 2011 Dec 2;286(48):41647-55.

 

Ferreres, F., Figueiredo, R., Bettencourt, S., Carqueijeiro, I., Oliveira, J., Gil-Izquierdo, A., Pereira, D., Valentão, P., Andrade, P.B., Duarte, P., Barceló, A., and Sottomayor, M. (2011). "Identification of phenolic compounds in isolated vacuoles of the medicinal plant Catharanthus roseus and their interaction with vacuolar class III peroxidase - an H2O2 affair?” Journal of Experimental Botany

 

Carolina Costa-Ramosa, Ana do Valea, Paula Ludovicob, Nuno M.S. dos Santosa and Manuel T. Silva “The bacterial exotoxin AIP56 induces fish macrophage and neutrophil apoptosis using mechanisms of the extrinsic and intrinsic pathways” Fish & Shellfish Immunology, Volume 30, Issue 1, January 2011, 173-181

 

Pires RH, Saraiva MJ, Damas AM, Kellermayer MS. Structure and assembly-disassembly properties of wild-type transthyretin amyloid protofibrils observed with atomic force microscopy. J Mol Recognit. 2011 May-Jun;24(3):467-76.

 

Rocha, R.; Pereira, P.J.B.; Santos, M.M.A.; Macedo-Ribeiro, S. (2011) “Purification, crystallization and preliminary X-ray diffraction analysis of the seryl-tRNA synthetase from Candida albicans.” Acta Crystallographica F67, 153-156

 

Mannarino SC, Vilela LF, Brasil AA, Aranha JN, Moradas-Ferreira P, Pereira MD, Costa V, Eleutherio EC. “Requirement of glutathione for Sod1 activation during lifespan extension.” Yeast. 2011 Jan;28(1):19-25.

 

Barbosa AD, Osório H, Sims KJ, Almeida T, Alves M, Bielawski J, Amorim MA, Moradas-Ferreira P, Hannun YA, Costa V. Role for Sit4p-dependent mitochondrial dysfunction in mediating the shortened chronological lifespan and oxidative stress sensitivity of Isc1p-deficient cells. Mol Microbiol. 2011 Jul;81(2):515-27.

 

Beites T, Pires SD, Santos CL, Osório H, Moradas-Ferreira P, Mendes MV. Crosstalk between ROS Homeostasis and Secondary Metabolism in S. natalensis ATCC  27448: Modulation of Pimaricin Production by Intracellular ROS. PLoS One 2011; 6(11):e27472

 

Magalhães J, Saraiva MJ. Clusterin overexpression and its possible protective role in transthyretin deposition in familial amyloidotic polyneuropathy. J Neuropathol Exp Neurol. 2011 Dec;70(12):1097-106.

 

Castro-Rodrigues AF, Gales L, Saraiva MJ, Damas AM. Structural insights into a zinc-dependent pathway leading to Leu55Pro transthyretin amyloid fibrils. Acta Crystallogr D Biol Crystallogr. 2011 Dec;67(Pt 12):1035-44.

 

Pessoa, J, Sárkány, Z., Martins, S., Ferreira-da-Silva, F., Almeida, MR and Damas, AM (2010) “Functional characterization of the Arabidopsis thaliana transthyretin-like protein”, BMC Plant Biology 10:30

 

Manning AL, Bakhoum SF, Maffini S, Correia-Melo C, Maiato H, Compton DA. “CLASP1, astrin and Kif2b form a molecular switch that regulates kinetochore-microtubule dynamics to promote mitotic progression and fidelity.” EMBO J. 2010 Oct 20;29(20):3531-43.

 

Fraga H, Faria TQ, Pinto F, Almeida A, Brito RM, Damas AM. “FH8--a small EF-hand protein from Fasciola hepatica.” FEBS J. 2010 Dec;277(24):5072-85.

 

Castro H, Romao S, Carvalho S, Teixeira F, Sousa C, Tomás AM. “Mitochondrial redox metabolism in trypanosomatids is independent of tryparedoxin activity” .PLoS One. 2010 Sep 8;5(9):e12607.

 

Macedo B, Magalhães J, Batista AR, Saraiva MJ, “Carvedilol treatment reduces transthyretin deposition in a familial amyloidotic polyneuropathy mouse model.” Pharmacol Res. 2010 Dec;62(6):514-22.

 

Seabra, A.R.; Carvalho, H.; Pereira, P.J.B. (2009) “Crystallization and preliminary crystallographic characterization of glutamine synthetase from Medicago truncatula.” Acta Crystallographica F65, 1309-1312.

 

Maffini S, Maia AR, Manning AL, Maliga Z, Pereira AL, Junqueira M, Shevchenko A, Hyman A, Yates JR 3rd, Galjart N, Compton DA, Maiato H. “Motor-independent targeting of CLASPs to kinetochores by CENP-E promotes microtubule turnover and poleward flux.” Curr Biol. 2009 Sep 29;19(18):1566-72.

 

Mariana Lince-Faria, Stefano Maffini, Bernard Orr, Yun Ding, Cláudia Florindo, Claudio E. Sunkel, Álvaro Tavares, Jørgen Johansen, Kristen M. Johansen, and Helder Maiato “Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator” J Cell Biol. 2009 March 9; 184(5): 647–657.

 

Carvalho S, Cruz T, Santarém N, Castro H, Costa V, Tomás AM. “Heme as a source of iron to Leishmania infantum amastigotes.” Acta Trop. 2009 Feb;109(2):131-5.

 

Costa R, Ferreira-da-Silva F, Saraiva MJ, Cardoso I. (2008) "Transthyretin protects against A-beta peptide toxicity by proteolytic cleavage of the peptide: a mechanism sensitive to the Kunitz protease inhibitor" PLoS ONE 3 (8): e2899

 

Helena Castro, Susana Romao, Fernanda R. Gadelha, Ana M. Tomás (2008) "Leishmania infantum: provision of reducing equivalents to the mitochondrial tryparedoxin/tryparedoxin peroxidase system" Experimental Parasitology DOI: 10.1016/j.exppara.2008.09.002

 

Santos SD, Costa R, Teixeira PF, Gottesman M, Cardoso I, Saraiva MJ (2008) "Amyloidogenic properties of transthyretin-like protein (TLP) from Escherichia coli" FEBS Lett. 582(19):2893-8

 

Mech Ageing Dev. 2008 Sep 18 Glutathione is necessary to ensure benefits of calorie restriction during ageing in Saccharomyces cerevisiae. Mannarino SC, Amorim MA, Pereira MD, Moradas-Ferreira P, Panek AD, Costa V, Eleutherio EC

 

Almeida T, Marques M, Mojzita D, Amorim MA, Silva RD, Almeida B, Rodrigues P, Ludovico P, Hohmann S, Moradas-Ferreira P, Côrte-Real M, Costa V. Isc1p plays a key role in hydrogen peroxide resistance and chronological lifespan through modulation of iron levels and apoptosis. Mol Biol Cell. 2008 Mar;19(3):865-76.

 

Members of the E2D (UbcH5) family mediate the ubiquitination of the conserved cysteine of Pex5p, the peroxisomal import receptor. Grou CP, Carvalho AF, Pinto MP, Wiese S, Piechura H, Meyer HE, Warscheid B, Sá-Miranda C, Azevedo JE. J Biol Chem. 2008 May 23;283(21):14190-7.

 

Mycobacterium tuberculosis Glucosyl-3-Phosphoglycerate Synthase: Structure of a Key Enzyme in Methylglucose Lipopolysaccharide Biosynthesis. Pereira PJ, Empadinhas N, Albuquerque L, Sá-Moura B, da Costa MS, Macedo-Ribeiro S. PLoS ONE. 2008;3(11):e3748.

 

Crystallization and preliminary crystallographic analysis of mannosyl-3-phosphoglycerate synthase from Rubrobacter xylanophilus. Sá-Moura B, Albuquerque L, Empadinhas N, da Costa MS, Pereira PJ, Macedo-Ribeiro S. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Aug 1;64(Pt 8):760-3

 

Isolation, cloning and structural characterisation of boophilin, a multifunctional Kunitz-type proteinase inhibitor from the cattle tick.Macedo-Ribeiro S, Almeida C, Calisto BM, Friedrich T, Mentele R, Stürzebecher J, Fuentes-Prior P, Pereira PJ. PLoS ONE. 2008 Feb 20;3(2):e1624

 

Marques I, Ushakova AV, Duarte M, Videira A. (2007) "Role of the conserved cysteine residues of the 11.5 kDa subunit in complex I catalytic properties" J Biochem. 2007 Apr;141(4):489-93

 

Belinha I, Amorim MA, Rodrigues P, de Freitas V, Moradas-Ferreira P, Mateus N and Costa V (2007) "Quercetin increases oxidative stress resistance and longevity in Saccharomyces cerevisiae ” J Agric Food Chem 55 , 2446

 

Carvalho AF, Grou CP, Pinto MP, Alencastre IS, Costa-Rodrigues J, Fransen M, Sá-Miranda C and Azevedo JE (2007) “Functional characterization of two missense mutations in Pex5p - C11S and N526K.” BBA – Mol Cell Res, 1773, 1141-1148.

 

Carvalho AF, Pinto MP, Grou CP, Alencastre IS, Fransen M, Sá-Miranda C and Azevedo JE (2007) “Ubiquitination of mammalian Pex5p, the peroxisomal import receptor.” J Biol Chem, 282, 31267-31272

 

Santos CL, Vieira J, Sellstedt A, Normand P, Moradas-Ferreira P and Tavares F. (2007) "Modulation of Frankia alni ACN14a oxidative stress response: activity, expression and phylogeny of catalases." Physiol Plant 130: 454-463.

 

F. Ferreira-da-Silva, P.J.B. Pereira, L. Gales, M. Roessle, D.I. Svergun, P. Moradas-Ferreira e A.M. Damas (2006) “The crystal and solution structures of glyceraldehyde-3-phosphate dehydrogenase reveal different quaternary structures.” J. Biol. Chem. 281 (44): 33433-33440

 

Carvalho, A.F., Costa-Rodrigues, J., Correia, I., Costa Pessoa, J., Faria, T.Q., Martins, C.L., Fransen, M., Sá-Miranda,C., and Azevedo,J.E. (2006). “The N-terminal half of the peroxisomal cycling receptor Pex5p is a natively unfolded domain”. J. Mol Biol. 356, 864-875

 

Teixeira, P.F., Cerca, F., Santos, S.D., Saraiva, M.J. (2006) “Endoplasmic reticulum stress associated with extracellular aggregates. Evidence from transthyretin deposition in familial amyloid polyneuropathy”. J. Biol Chem. 281(31), 21998-2003

 

Pinto, M.P., Grou, C.P., Alencastre, I.S., Oliveira, M.E., Sa-Miranda, C., Fransen, M., Azevedo, J.E. (2006) “The import competence of a peroxisomal membrane protein is determined by Pex19p before the docking step”. J. Biol Chem. 281(45), 34492-502.

 

Marques M, Mojzita D, Amorim MA, Almeida T, Hohmann S, Moradas-Ferreira P, Costa V. The Pep4p vacuolar proteinase contributes to the turnover of oxidized proteins but PEP4 overexpression is not sufficient to increase chronological lifespan in Saccharomyces cerevisiae. Microbiology. 2006 Dec;152(Pt 12):3595-605

 

Ushakova AV, Duarte M, Vinogradov AD, Videira A (2005) "The 29.9 kDa subunit of mitochondrial complex I is involved in the enzyme active/de-active transitions" J Mol Biol. 351(2):327-33

 

Costa-Rodrigues J, Carvalho AF, Fransen M, Hambruch E, Schliebs W, Sá-Miranda C, Azevedo JE. (2005) "Pex5p, the peroxisomal cycling receptor, is a monomeric non-globular protein" J. Biol Chem. 280(26), 24404-11.

 

Duarte M, Schulte U, Ushakova AV, Videira A. (2005) "Neurospora strains harboring mitochondrial disease-associated mutations in iron-sulfur subunits of complex I" Genetics. 171(1):91-9